Effect on hyperplasia of VSMCS in rat by the plasmid containing the short hairpin RNA of AT1R / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 263-267, 2004.
Article
en Zh
| WPRIM
| ID: wpr-330125
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>RNA interference is a new technology that inhibit effectively of the expression the specific genes. The present study was designed to investigate whether the plasmid containing the short hairpin RNA (shRNA) of angiotensin II type 1 receptor (AT1R) can inhibit the hyperplasia of VSMCs in rat.</p><p><b>METHODS</b>The plasmids containing the shRNA of AT1R were constructed, and transfected vascular smooth muscle cell (VSMC) to detect the effect on the AT1R expression by RT-PCR and Western blot, and detect the hyperplasia of VSMCs by trypan blues training and MTT.</p><p><b>RESULTS</b>The plasmids was certified to be in the right rank, and there was significant difference (P < 0.01) in the expression of AT1R mRNA and protein between the plasmid transfected group and the control group. There was significant difference (P < 0.01) in the hyperplasia of VSMCs between the plasmid transfected adding Ang II group and the control group.</p><p><b>CONCLUSION</b>The plasmids containing the shRNA of AT1R have the effect of RNAi, and inhibit the hyperplasia of VSMCs induced by Ang II in rat.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
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Células Cultivadas
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Ratas Wistar
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Biología Celular
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Miocitos del Músculo Liso
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ARN Interferente Pequeño
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Interferencia de ARN
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Receptor de Angiotensina Tipo 1
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Proliferación Celular
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2004
Tipo del documento:
Article