Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP) / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 403-405, 2008.
Article
en Zh
| WPRIM
| ID: wpr-332485
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.</p><p><b>METHODS</b>Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.</p><p><b>RESULTS</b>The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.</p><p><b>CONCLUSION</b>RT-LAMP is more sensitive than nest-RT-PCR.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virología
/
Genoma Viral
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Sarampión
/
Virus del Sarampión
/
Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article