Gene mutation in the areas of PreC/C and BCP of HBV DNA / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 68-70, 2005.
Article
en Zh
| WPRIM
| ID: wpr-333046
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the gene mutation in the areas of pre core/core (Pre C/C) and basic core promotor (BCP) of HBV DNA and its clinical significance.</p><p><b>METHODS</b>The nt 1 735-1 965 segment of HBV DNA was amplified with PCR in 54 cases with chronic hepatitis B and 10 cases with post-hepatitis cirrhosis. Then the PCR product was sequenced.</p><p><b>RESULTS</b>There were 168 site mutations in 48.5% (33/68) cases with hepatitis B. The first ten mutation sites were nt 1 764 (58.8%), 1 762 (48.5%), 1 799 (21.0%), 1 766 (14.7%), 1 896 (13.2%), 1 754 (8.8%), 1 899 (8.8%), 1 768 (7.4%), 1 814 (7.4%) and 1 913 (7.4%). Three rare mutations of nt 1907, 1 922 and 1 923 were also detected. The mutations of nt 1 896, 1 764 and 1 762 were found in 16.7%, 35.2% and 35.2% of chronic hepatitis, and in 30.0%, 60.0% and 60.0% respectively of post-hepatitis cirrhosis cases. There was statistical significance between the two groups (P < 0.01).</p><p><b>CONCLUSION</b>The mutations in the areas of Pre C/C and BCP of HBV DNA might possibly be associated with liver fibrosis. There are many mutation sites in HBV DNA and mutation occurs frequently, therefore gene sequencing is helpful to the design of gene chip and to clinical application.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Virología
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Sangre
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Polimorfismo de Longitud del Fragmento de Restricción
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ADN Viral
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Virus de la Hepatitis B
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Reacción en Cadena de la Polimerasa
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Mutación Puntual
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Hepatitis B Crónica
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Frecuencia de los Genes
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Article