Silencing of survivin gene enhances chemosensitivity of human tongue cancer cell line Tca8113 to cisplatin / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 280-283, 2007.
Article
en Zh
| WPRIM
| ID: wpr-333343
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of survivin short hairpin RNA (shRNA) on survivin expression, cell apoptosis, and chemosensitivity of human tongue cancer cell Tca8113 to cisplatin.</p><p><b>METHODS</b>Survivin-directed shRNA plasmid vector was delivered into Tca8113 cells with lipofectamine(TM) 2000 reagent. Survivin expression was detected with the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Flow cytometry was used to examine cell apoptosis, and the sensitivity to anticancer agents was evaluated by methyl thiazolyl tetrazolium (MTT) assay.</p><p><b>RESULTS</b>After survivin shRNA vector transfection in Tca8113 cells, the expression of mRNA/protein declined significantly, and the apoptotic rate increased in time-dependent manner up to 37.9% at 48 hours. RNAi-mediated survivin reduction selectively inhibited growth and enhanced chemosensitivity of cisplatin but not of 5-fluorouracil.</p><p><b>CONCLUSIONS</b>Survivin shRNA could inhibit the expression of survivin mRNA and it's protein and enhance the chemosensitivity of cisplatin.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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ARN Mensajero
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Neoplasias de la Lengua
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Carcinoma de Células Escamosas
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Transfección
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Cisplatino
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Apoptosis
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Resistencia a Antineoplásicos
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Interferencia de ARN
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Año:
2007
Tipo del documento:
Article