Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1520-1527, 2015.
Article
en Zh
| WPRIM
| ID: wpr-337469
Biblioteca responsable:
WPRO
ABSTRACT
1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Glicoles de Propileno
/
Microbiología Industrial
/
Propilenglicol
/
Medios de Cultivo
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Fermentación
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Glicerol
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Klebsiella pneumoniae
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Metabolismo
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Métodos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Article