Preparation of ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong by preparative HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1947-1950, 2013.
Article
en Zh
| WPRIM
| ID: wpr-346465
Biblioteca responsable:
WPRO
ABSTRACT
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (50:50)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Benzofuranos
/
Química
/
Cromatografía Líquida de Alta Presión
/
Ácidos Cumáricos
/
Ligusticum
/
Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2013
Tipo del documento:
Article