Alterations in cardiac structure and function in a modified rat model of myocardial hypertrophy / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 626-633, 2014.
Article
en En
| WPRIM
| ID: wpr-351027
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to establish a stable animal model of left ventricular hypertrophy (LVH) to provide theoretical and experimental basis for understanding the development of LVH. The abdominal aorta of male Wistar rats (80-100 g) was constricted to a diameter of 0.55 mm between the branches of the celiac and anterior mesenteric arteries. Echocardiography using a linear phased array probe was performed as well as pathological examination and plasma B-type natriuretic peptide (BNP) measurement at 3, 4 and 6 weeks after abdominal aortic constriction (AAC). The results showed that the acute mortality rate (within 24 h) of this modified rat model was 8%. Animals who underwent AAC demonstrated significantly increased interventricular septal (IVS), LV posterior wall (LVPWd), LV mass index (LVMI), cross-sectional area (CSA) of myocytes, and perivascular fibrosis; the ejection fraction (EF), fractional shortening (FS), and cardiac output (CO) were consistently lower at each time point after AAC. Notably, differences in these parameters between AAC group and sham group were significant by 3 weeks and reached peaks at 4th week. Following AAC, the plasma BNP was gradually elevated compared with the sham group at 3rd and 6th week. It was concluded that this modified AAC model can develop LVH, both stably and safely, by week four post-surgery; echocardiography is able to assess changes in chamber dimensions and systolic properties accurately in rats with LVH.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Aorta Abdominal
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Patología
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Factores de Tiempo
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Sangre
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Ensayo de Inmunoadsorción Enzimática
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Ecocardiografía
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Ratas Wistar
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Hipertrofia Ventricular Izquierda
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Constricción Patológica
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Péptido Natriurético Encefálico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2014
Tipo del documento:
Article
Pais de publicación:
CHINA
/
CN
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REPUBLIC OF CHINA