Analysis of pulsed-field gel electrophoresis molecular subtyping of Shigella strains in Shenzhen / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 317-320, 2008.
Article
en Zh
| WPRIM
| ID: wpr-352480
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the genetic relations of Shigella isolated from Shenzhen in 2001-2006 and develop primary molecular subtyping surveillance network of Shigella.</p><p><b>METHODS</b>Chromosomal DNAs from 55 isolated in agarose were digested with the restriction enzyme Xba I, and then were analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns were clustered using BioNumerics software.</p><p><b>RESULTS</b>All 41 distinctive PFGE patterns were identified among 55 strains. 32 strains belonged to one cluster. Differences were observed in other strains.</p><p><b>CONCLUSION</b>Both genetic-related clones and non-related clones of Shigella existed in Shenzhen. The development of PFGE molecular subtyping surveillance network would contribute to the active surveillance, outbreak investigation and source tracking for Shigellosis.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Shigella
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China
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Técnicas de Tipificación Bacteriana
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Clasificación
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Electroforesis en Gel de Campo Pulsado
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Heces
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Métodos
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Microbiología
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Año:
2008
Tipo del documento:
Article