Establishment of an indirect enzyme-linked immunosorbent assay for detecting the specific IgM antibodies in patients with echovirus infection / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To provide a simple, specific and early serodiagnostic technique for the patients with aseptic meningitis caused by echovirus.</p><p><b>METHODS</b>An indirect enzyme-linked immunosorbent assay (ELISA) has been developed to detect echovirus-IgM and the specificity and availability of the assay were also examined.</p><p><b>RESULTS</b>In 78 cerebrospinal fluid (CSF) specimens which came from the children with aseptic meningitis, the positive rate was 17.9(14/78). In 64 CSF collected from non-aseptic meningitis (bacterial meningitis and cerebral trauma), the positive rate was 1.56(1/64). In 5 CSF specimens which were ELISA positive, the positive rate of neutralization test (NT) was 4/5, all the specimens which were ELISA negative were NT negative. In this assay there was no cross-reaction with poliovirus, Coxsackie virus B type 1-6 and A type 7. By blocking and destructive test of specific IgM, all CSF specimens with ELISA positive became negative.</p><p><b>CONCLUSIONS</b>The established indirect ELISA was specific and reliable. The te st was quick, simple and available, which is suitable for early and specific clinical diagnosis, and will be greatly significant to clinical treatment.</p>