Carbon monoxide inhalation protects against limb ischemia/reperfusion injury / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 33-36, 2010.
Article
en Zh
| WPRIM
| ID: wpr-356224
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of carbon monoxide(CO) inhalation in the limb ischemia/reperfusion (I/R) injury of rats.</p><p><b>METHODS</b>Forty-four Sprague-Dawley rats were randomly divided into three groups: S, I/R and RC groups. I/R injury model was made by the occlusion of the femoral artery for four hours and the reperfusion for forty-eight hours. RC group was exposed to medical air mixed CO (the volume fraction of CO: 0.05%) during limb reperfusion in rats. The other two groups were exposed to the routine air. Gross morphology of the ischemic limb, wet-to-dry weight ratio (W/D), and skeletal muscle histopathology were observed. The apoptosis index and expression levels of Bax and Bcl-2 in the muscle were assessed with Flow Cytometry. The activities of serum lactate dehydrogenase (LDH) and creatine kinase (CK) were tested by Automatic Biochemical Analyzer.</p><p><b>RESULTS</b>Compared to I/R group, W/D, serum LDH and CK activities, the apoptosis index and Bax expression level in the muscle were all significantly decreased, the Bcl-2 expression level was significantly increased, gross morphology of the ischemic limb and muscle histopathology were obviously improved in RC group.</p><p><b>CONCLUSION</b>Inhaling exogenous CO can attenuate limb I/R injury.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Administración por Inhalación
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Monóxido de Carbono
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Daño por Reperfusión
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Ratas Sprague-Dawley
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Proteínas Proto-Oncogénicas c-bcl-2
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Creatina Quinasa
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Proteína X Asociada a bcl-2
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Extremidades
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Arteria Femoral
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2010
Tipo del documento:
Article