Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation.</p><p><b>METHODS</b>16 male SD rats were separated into 2 groups (n=8) 2 kidney 1 Clip group (2K1C) and sham operation group (SO). The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).</p><p><b>RESULTS</b>Overall, 21 protein spots showed significant difference, and 14 out of which were identified.</p><p><b>CONCLUSION</b>Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.</p>