Effect of genistein on the expression of Bax and Bcl-2 protein in PC12 cells transfected with mutant APP695 type / 中华行为医学与脑科学杂志
Chinese Journal of Behavioral Medicine and Brain Science
; (12): 791-793, 2013.
Article
en Zh
| WPRIM
| ID: wpr-441913
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of genistein on the expression of Bax and Bcl-2 protein in PC12 cells transfected with mutant APP695 Type.Methods PC12 cells were transfected with pIRES2-EGFP plasmid or pIRES2-EGFP/APP695MT expression plasmid,and then divided into normal control group,Unladen group,APP695 transfected group,GST treatment group(5 μmol/L,15 μmol/L).The Bax and Bcl-2 protein expression in PC12 cells was measured by Immunocytochemistry SABC and Western-blot.Results Immunocyto-chemistry SABC showed thatcompared with the normal control group,the expression of bax protein in APP695 transfected group was strongly increased((78.02 ± 1.26) %,P<0.01)) and the expression of bcl-2 protein was very weak oppositely ((15.40 ± 0.72) %,P<0.01)).Compared with APP695 transfected group,the expression of bax protein in cells of 15umol/L GST treatment group was significantly decreased((22.35 ± 0.49) %,P<0.01))and the expression of bcl-2 protein in these cells was strongly increased ((68.11 ± 0.24) %,P <0.01)).Western-Blot showed thatcompared with the normal control group,the expression of bax protein in APP695 transfected group was markedly increased and the expression of Bcl-2 protein was significantly decreased.Compared with the APP695 transfected group,the expression of Bax protein was markedly decreased and the expression of Bcl-2 protein was significantly increased in the cells of the GST treatment groups.Conclusion GST can reduce the expression of bax protein and the increased expression of Bcl-2 protein has protective effect on PC12 cells transfected with APP696MT.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Behavioral Medicine and Brain Science
Año:
2013
Tipo del documento:
Article
Pais de publicación:
CHINA
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CN
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REPUBLIC OF CHINA