The correlation of vitamin D level and vitamin D-binding protein gene polymorphism in chronic obstructive pulmonary disease / 中华内科杂志
Chinese Journal of Internal Medicine
; (12): 303-307, 2014.
Article
en Zh
| WPRIM
| ID: wpr-443395
Biblioteca responsable:
WPRO
ABSTRACT
Objective To assess the correlation of serum 25-hydroxyvitamin D (25-OHD) levels with vitamin D-binding protein (the group-specific component,GC) gene polymorphism in chronic obstructive pulmonary disease (COPD).Methods In a cross-sectional case-control study,250participants,including 116 COPD patients with smoking history and 134 healthy smokers,were investigated.A questionnaire about smoking history,vitamin D intake and comorbidities was collected.General pulmonary function was done by routine.Serum 25-OHD levels were detected by ELISA.The genetic variants (rs4588and rs7041) were genotyped by real time fluorescence polymerase chain reaction (RT-PCR) with TaqMan probe technology.Results The COPD patients had lower serum vitamin D level than the smoker subjects (36.58 nmol/L vs 43.80 nmol/L,P <0.001).In the COPD patients,vitamin D level was 39.43 nmol/L in those with percentage of predicted forced expiratory volume in 1 second (FEV1 % pred) greater than or equal to 80%.In other groups with FEV1 % pred 50%-80%,30%-50% and lower than 30%,vitamin D levels were 35.32 nmol/L,32.21 nmol/L,26.25 nmol/L respectively (P < 0.01).Moreover,there was a significant relevance of 25-OHD levels with FEV1 % pred in both COPD patients and healthy smokers (r2 =1.911; P <0.000 1).The mean 25-OHD concentration had a negative correlation with Global Initiative for Obstructive Lung Disease (GOLD) stages.Homozygous carriers of vitamin D-binding protein gene rs7041 T allele were independently related to 25-OHD levels and susceptibility of COPD (P < 0.01 ; OR =2.140,95% CI 1.157-3.959,P =0.015 respectively).Conclusions Patients with COPD are at high risk of vitamin D deficiency and the severity of COPD is inversely correlated with vitamin D levels.Furthermore,homozygous carrier of rs7041 T allele influences 25-OHD serum levels and is related to susceptibility of COPD,which may be a potential candidate gene for screening COPD.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Idioma:
Zh
Revista:
Chinese Journal of Internal Medicine
Año:
2014
Tipo del documento:
Article