Identification of IgE-reacting Clonorchis sinensis antigens
Yonsei Medical Journal
; : 178-183, 1999.
Article
en En
| WPRIM
| ID: wpr-45255
Biblioteca responsable:
WPRO
ABSTRACT
Clonorchis sinensis is a liver fluke and it is the most prevalent human parasite in Korea at present. The parasite infection induces immune responses, characteristically an increased production of parasite-specific IgE in the host. Major IgE-reacting C. sinensis antigens in infected humans have been protein bands with MWs of 15, 28, 37, 45, 51, 56, 62, 66, 74, 97 and 160 KD identified by immunoblot analysis. Individual variations of the IgE binding pattern to C. sinensis antigens have also been documented. Using immune BALB/c mouse sera, IgE-reacting protein bands have been visualized with MWs of 28, 74, 86, 160 and several > 200 KD. One of the most strongly reacted C. sinensis antigenic proteins with a molecular weight of 28 KD was purified by gel filtration and preparative electrophoresis. Using a monoclonal antibody produced against the antigenic protein, the protein was localized in the parasite's intestine, and also found to be contained in excretory-secretory antigens.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Inmunoglobulina E
/
Immunoblotting
/
Técnica del Anticuerpo Fluorescente
/
Clonorchis sinensis
/
Animales
/
Ratones Endogámicos BALB C
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Anticuerpos Monoclonales
/
Antígenos Helmínticos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Yonsei Medical Journal
Año:
1999
Tipo del documento:
Article