Analysis of miRNA-34c expression in cervical cancer tissue and preliminary identification of Polo-like kinase 4 as its target gene / 中国肿瘤临床
Chinese Journal of Clinical Oncology
; (24): 1077-1080, 2015.
Article
en Zh
| WPRIM
| ID: wpr-482938
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To analyze the expression profile of miRNA-34c in cervical cancer tissue and identify its novel target gene.Methods:
The expression levels of miRNA-34c were detected in 34 paired cervical cancer tissues and normal paraneoplastic tissues via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Polo-like kinase 4 (PLK4) is a target gene of miRNA-34c pre-dicted in the miRNA Target Database. Luciferase vector containing the binding site of miRNA-34c to PLK4 3'UTR and miRNA-34c mimic or negative control were co-transfected into HEK293T cells, and luciferase expression was examined. The miRNA-34c mimic or negative control was transfected into SiHa cells, and the mRNA or protein expression of PLK4 was detected via qRT-PCR or Western blot, respectively.Results:
MiRNA-34c expression was lower in cervical cancer tissues than in normal paraneoplastic tissues. The miR-NA-34c mimics significantly inhibited luciferase activation in the HEK293T cells (P<0.01) and significantly decreased the mRNA and protein expression levels of PLK4 in the SiHa cells (P<0.01).Conclusion:
MiRNA-34c is significantly decreased in cervical cancer tis-sues. Moreover, miRNA-34c can significantly repress the mRNA and protein expression levels of PLK4 by directly targeting the 3'UTR.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Clinical Oncology
Año:
2015
Tipo del documento:
Article