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Analysis of the Genetic Diversity of Germplasm Resources of Duchesnea indica from Hunan by Random Amplified Polymorphic DNA / 中国药房
China Pharmacy ; (12): 2628-2631, 2015.
Article en Zh | WPRIM | ID: wpr-500923
Biblioteca responsable: WPRO
ABSTRACT

OBJECTIVE:

To study the genetic diversity of germplasm resources of Duchesnea indica from Hunan.

METHODS:

The random amplified polymorphic DNA(RAPD)reaction system of germplasm resources was established and RAPD-PCR was ad-opted to detect the expressions of 24 groups of D. indica from Hunan(including 21 wild and 3 cultivated varietos in different geo-graphical distribution). Agarose gel electrophoresis was used to analyze the results and calculate allele number (Na),effective al-lele number (Ne) the polymorphism points percentage (PPB),Nei’s gene diversity index (H) and Shannon’s information index (I).

RESULTS:

Totally 14 polymorphic primers were screened and 90 electrophoresis bands were amplified,including 81 polymor-phic bands with Na of 1.900 0,Ne of 1.540 1,PPB of 90.0%,H of 0.312 8 and I of 0.467 5.

CONCLUSIONS:

The genetic diver-sity of D. indica from Hunan is rich,and there were differences in genetic background between the cultivated species and wild spe-cies;clustering results show significant correlation with geographic distribution.
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Texto completo: 1 Base de datos: WPRIM Tipo de estudio: Clinical_trials Idioma: Zh Revista: China Pharmacy Año: 2015 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Tipo de estudio: Clinical_trials Idioma: Zh Revista: China Pharmacy Año: 2015 Tipo del documento: Article