Analysis of the Genetic Diversity of Germplasm Resources of Duchesnea indica from Hunan by Random Amplified Polymorphic DNA / 中国药房
China Pharmacy
; (12): 2628-2631, 2015.
Article
en Zh
| WPRIM
| ID: wpr-500923
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:
To study the genetic diversity of germplasm resources of Duchesnea indica from Hunan.METHODS:
The random amplified polymorphic DNA(RAPD)reaction system of germplasm resources was established and RAPD-PCR was ad-opted to detect the expressions of 24 groups of D. indica from Hunan(including 21 wild and 3 cultivated varietos in different geo-graphical distribution). Agarose gel electrophoresis was used to analyze the results and calculate allele number (Na),effective al-lele number (Ne) the polymorphism points percentage (PPB),Nei’s gene diversity index (H) and Shannon’s information index (I).RESULTS:
Totally 14 polymorphic primers were screened and 90 electrophoresis bands were amplified,including 81 polymor-phic bands with Na of 1.900 0,Ne of 1.540 1,PPB of 90.0%,H of 0.312 8 and I of 0.467 5.CONCLUSIONS:
The genetic diver-sity of D. indica from Hunan is rich,and there were differences in genetic background between the cultivated species and wild spe-cies;clustering results show significant correlation with geographic distribution.
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Base de datos:
WPRIM
Tipo de estudio:
Clinical_trials
Idioma:
Zh
Revista:
China Pharmacy
Año:
2015
Tipo del documento:
Article