Content Determination of L-epicatechin in Actinidia arguta by HPLC / 中国药房
China Pharmacy
; (12): 378-380, 2016.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-501425
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of L-epicatechin in Actinidiae arguta.METHODS:
HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2%Acetic acid solution(1585,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 25 ℃,and the volume injection was 10 μl.RESULTS:
The linear range of L-epicatechin was 10.47-167.52 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;average recovery was 98.07%-101.71%(RSD=1.39%,n=6).CONCLUSIONS:
The method is simple, accurate and reliable,and suitable for the content determination of L-epicatechin in A. arguta.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2016
Tipo del documento:
Artículo