Determination of serum free testosterone concentration:a comparison of Vermeulen calculation and enzyme immunoassay results / 中华泌尿外科杂志

ABSTRACT

ObjectiveTo compare the results of Verme ul en calculation and the enzyme immunoassay in the determination of serum free tes tosterone (FT)concentration in aging males.Methods129 healthy males over 45 years of age who have no disease or medicine that can infl uence the secretion of hormone were enrolled in the study. They were divided int o 4 age groups. The serum specimens were prepared and kept under -40 ℃ until as saying. All the samples were divided into two parts, one measured by enzyme immu noassay and the other calculated with the Vermeulen formula after determining th e total testosterone(T)and sex-hormone-binding globulin(SHBG)FT=T- 23.43FTSHBG-(T-23.43FT)?10 -9 mol/L.The data were analyzed by S PSS.ResultsThe results of FT in the 129 males were (46 .69?21.79)pmol/L by enzyme immunoassay and (396.30?317.04)pmol/L by calculatio n.Pairing test suggested that " t " was 13.01 and the difference of FT w as remarkably significant ( P