Isolation and identification of rat FasL-expressing Sertoli cells / 解放军医学杂志

ABSTRACT

Objective To isolate and identify the Sertoli′ cells, and the expression of FasL was examined immunohistochemically. Methods Testis were isolated from male Wistar rats and Sertoli′ cells were isolated. After culturing for 72 hours, Sertoli′ cells were morphologically observed by several ways and identified with transmission electron microscope. FasL was examined immunohistochemically. Results The yielded Sertoli′ cells accounted for over 90% of all the cells harvested. FasL was expressed in high level in the 72h cultured Sertoli′ cells. Conclusion Sertoli′ cells that we have isolated can be used for co-transplantation with other cells and offer immune privilege.