Analysis of Mycoplasma Contamination in Donated Cord Blood Units / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 9-14, 2008.
Article
en Ko
| WPRIM
| ID: wpr-57132
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Mycoplasma spp. occasionally colonize the genital tract and these organisms are some of the most important contaminants in cell culture laboratories and cell banks. We analyzed the Mycoplasma contamination rates in the donated cord blood units (CBUs) before cell processing. METHODS: A total of 151 CBUs that were donated with informed consent (November 3rd~December 28th, 2006) were randomly selected and enrolled in the study. We performed blood culture and Mycoplasma DNA PCR assay with using samples from the collection bags before processing. RESULTS: All of the CBUs were obtained from full-term (gestational age 37~42 weeks) deliveries. Two units showed positive results on blood culture however, Mycoplasma DNA is not found in the tested samples. CONCLUSION: The contamination rates of Mycoplasma in the CBUs, which are donated from the mothers who have full-term delivery and no pregnancy complications, are extremely low. The donated CBUs could be used in culture and for an expansion process without concern of incurring pre-processing Mycoplasma contamination. The rate of Mollicute contamination in the CBUs could become clear with the results of performing Ureaplasma assay.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Complicaciones del Embarazo
/
Ureaplasma
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ADN
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Reacción en Cadena de la Polimerasa
/
Colon
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Técnicas de Cultivo de Célula
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Sangre Fetal
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Consentimiento Informado
/
Madres
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Mycoplasma
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Blood Transfusion
Año:
2008
Tipo del documento:
Article