Determination of Chlorogenic Acid in Liyan Granule by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish a HPLC method for determination of Chlorogenic acid in Liyan Granule. Method The determination was performed on Diamondsil Cl8 (4.6 mm?150 mm, 5 ?m) with mobile phase consisted of acetonitrile-0.4% phosphonic acid solution (13∶87) at a flow rate of 1.0 mL/min. The detective wavelength was set at 327 nm. Result The linear range of Chlorogenic acid was 0.026～0.26 ?g (r =0.999 9) and the average recovery rate of Chlorogenic acid was 101.84% (RSD=0.48%, n =6). Conclusion This method is simple, accurate and with good stabilization, and suitable for the quality control of Liyan gramule.