Discrepancy in Genotyping of Apolipoprotein E between Allele-Specific PCR and Fluorescence Resonance Energy Transfer or Sequencing / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 325-328, 2010.
Article
en En
| WPRIM
| ID: wpr-58467
Biblioteca responsable:
WPRO
ABSTRACT
The human apolipoprotein E (APOE) gene contains several single-nucleotide polymorphisms (SNPs) that are distributed across the gene. The genotype of the APOE gene has important implications as a risk factor for various diseases. We observed 2 cases in which the results of allele-specific PCR (AS-PCR) of the APOE gene were not consistent with those of fluorescence resonance energy transfer (FRET) or sequencing analysis. In these cases, genotyping by AS-PCR showed that patients were epsilon2 homozygotes, while sequencing analysis and FRET showed that they were epsilon2/epsilon3 heterozygotes. Herein, we describe the causes of the errors in genotyping and describe the significance of these errors.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Apolipoproteínas E
/
Reacción en Cadena de la Polimerasa
/
Factores de Riesgo
/
Análisis de Secuencia de ADN
/
Polimorfismo de Nucleótido Simple
/
Transferencia Resonante de Energía de Fluorescencia
/
Alelos
/
Apolipoproteína E2
/
Apolipoproteína E3
/
Genotipo
Tipo de estudio:
Etiology_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
The Korean Journal of Laboratory Medicine
Año:
2010
Tipo del documento:
Article