A study on preparation of recombinant human cystatin C and its polyclonal antibody / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1233-1234,1237, 2014.
Article
en Zh
| WPRIM
| ID: wpr-599029
Biblioteca responsable:
WPRO
ABSTRACT
Objective To explore the preparation of recombinant human cystatin C (Cys C ) and its polyclonal antibody . Methods The gene sequences encoding Cys C were designed according to characteristics of E .coli protein-coding ,and the target gene was artificially synthesized and expressed in E .coli .The target protein was purified by affinity chromatography and was used to immunize the New Zealand white rabbits .Western blot and enzyme-linked immunosorbent assay(ELISA) were adopted to detect the recombinant human Cys C and its polyclonal antibodies in the rabbits serum .Results Purity of prepared recombinant human Cys C was more than 90% .Anti-recombinant human Cys C antibody showed good binding activities and specificity .Conclusion Prepared recombinant human Cys C and its antibody can be used in further experimental research and clinical application .
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
International Journal of Laboratory Medicine
Año:
2014
Tipo del documento:
Article