Effect of long non-coding RNA HOTAIR on the proliferation and apoptosis of renal cancer cells / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 1095-1098,1099, 2015.
Article
en Zh
| WPRIM
| ID: wpr-601396
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of long non-coding RNA (LncRNA) Hox transcript antisense inter-genic RNA ( HOTAIR ) on the cell proliferation and apoptosis of renal cancer cell lines 786-O and ACHN. Methods Small interfere RNA ( siRNA ) that aims to down-regulate HOTAIR expression was transfected into two renal cell lines respectively, and the transfection efficiency was evaluated by qRT-PCR. Then the MTT assay,Ho-chest staining assay and enzyme-linked immunosorbnent assay(ELISA) were used to detect cell proliferation and apoptosis. Results The expression of HOTAIR could be down-regulated effectively by the siRNA (P < 0. 05). Down-regulation of HOTAIR could inhibit the proliferation(P < 0. 05) and increase apoptosis(P < 0. 05) of two re-nal cancer cells. Conclusion HOTAIR plays a role in promoting cell growth of renal cancer.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Universitatis Medicinalis Anhui
Año:
2015
Tipo del documento:
Article