Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 745-749, 2017.
Article
en Zh
| WPRIM
| ID: wpr-616551
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of electroacupuncture (EA) pretreatment on neurological function, and the expression of Toll-like receptor 4 (TLR4) and nuclear factorκB (NF-κB) protein in ischemic penumbra after cerebral ischemia-reperfusion (CIR) injury. Methods A total of 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and EA group (n=12). The later two groups were occluded their right middle cerebral arteries for two hours and reperfused. The EA group was pretreated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS), the injury in ischemic brain was detected with HE staining, and the expression of TLR4 and NF-κB protein in ischemic penumbra were detected with Western blotting, 24 hours after reperfusion. Results The score of mNSS decreased (P<0.05), the injury of brain tissue ameliorated, and the expression of TLR4 and NF-κB decreased in ischemic penumbra (P<0.05) in EA group compared with those in the model group. Conclu-sion EA pretreatment can reduce the injury and improve the neurological function in rats after CIR by down-regulating the expression of TLR4 and NF-κB protein in ischemic penumbra.
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1
Base de datos:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Año:
2017
Tipo del documento:
Article