Effects of glucocorticoids on intracellular calcium in microglial cells / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 878-883, 2017.
Article
en Zh
| WPRIM
| ID: wpr-618980
Biblioteca responsable:
WPRO
ABSTRACT
Aim To explore the effects of hydrocortisone on intracellular calcium in microglial cells.Methods The intracellular calcium was measured by instantaneous scanning with confocal laser microscope(CLM) in BV-2 cells, and fluo3-AM was used to dye the intracellular calcium.Results Both hydrocortisone and nicotine could obviously increase intracellular calcium in BV-2 cells(P0.05);but the blocker of α7 nicotinic acetylcholine receptor(α7nAChR) methyllycaconitine could suppress the rising of intracellular calcium induced by hydrocortisone(P<0.05).Conclusion Hydrocortisone enhances intracellular calcium via α7nAChR in microglial cells, which not only demonstrates the non-genomic effect of glucocorticoid, but also suggests that glucocorticoid could serve as endogenous ligand of α7nAChR.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Año:
2017
Tipo del documento:
Article