Tick diversity and detection of Coxiella burnetii in tick of small ruminants using nested Trans PCR in Southeast Iran

ABSTRACT

Ticks are obligatory bloodsucking arthropods, and probably the most harmful ectoparasites that may cause some tick born diseases. The main objective of this study was to determine the prevalence, diversity and seasonal distribution of ticks and using a nested Trans PCR to identify Coxiella burnetii in ticks collected from small ruminants in Sistan and Balouchestan province, southeast Iran. A total 1305 ticks were collected from 272 Sheep and 253 Goats during May 2014 to April 2015. Prevalence of ixodid tick infestation in small ruminants was 58.4%. Of all examined ticks, nine tick species were identified as follow: Hyalomma anatolicum anatolicum (30.3%), Rhipicephalus sanguineus (21%), Hyalomma anatolicum excavatum (19%), Rhipicephalus turanicus (9%), Rhipicephalus bursa (6.7%), Hyalomma detritum (4.7%), Hyalomma dromedarii (4.4%), Hyalomma asiaticum asiaticum (4.4%) and Hyalomma marginatum (0.5%). The nested Trans PCR examination of ixodid ticks revealed that Hyalomma anatolicum anatolicum and Rhipicephalus sanguineus were infected with C. burnetii. The results of the present study revealed that ixodid ticks infestation was widespread and shows their role as putative vectors and reservoirs for this pathogenic agent in southeast Iran. Hence; Q fever should be considered a significant public health threat in this region.