Effects of hVEGF genetically engineering membrane on the expression of flt-1 and TSP-1 during the healing process of full-thickness cutaneous defect / 上海第二医科大学学报

ABSTRACT

Objective To study the effects of hVEGF on the expression of fms-like tyrosine kinase-1(flt-1)and thrombospondin 1(TSP-1)during the healing process of rats' full-thickness cutaneous defect which covered by genetically membrane planted by fibroblast integrated with hVEGF recombinant.Methods Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats.Eighty rats were divided into four groups at random,20 rats each.For the rats in the experimented group A,the wounds were covered with hVEGF genetically engineering membrane and Tegaderm membrane.In the control group B,the wounds were covered with membrane planted by the fibroblasts integrated with blank plasmid and Tegaderm membrane.In the control group C,the wounds were covered with blank membrane and Tegaderm membrane.In control group D,the wounds were only covered with Tegadern membrane.Specimens were obtained at the 3,7,14 and 29 days after injury,immunohistochemistry and image analysis were used to observe the expression of flt-1 and TSP-1.Results The expression of flt-1 in vascular endothelial cells was increased obviously in the experimental group.There was significant difference between the experimental group and other groups at 3,7 and 14d after injury(P