Biological Activity and Single Oral Dose Toxicity of Dansam-samultang / 한국실험동물학회지

ABSTRACT

In this study, we investigated the in vitro antioxidative effects, antimicrobial activities and single oral dose toxicity of the extracts from Dansam-samultang to evaluate its use as a functional ingredient in cosmetics. In the antioxidative effect, the ethanol extract from Dansam-samultang (DSE) had higher antioxidant values of 92.0% at 1,000 microg/mL than that of water extract from Dansam-samultang (DSW, 86.0%) when evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The superoxide dismutase-like activity of DSW and DSE were 16.3% and 21.3% at 1,000 microg/mL in concentration, respectively. Xanthine oxidase inhibition activity of the DSE was higher 51.5% than that of the DSW (21.4%). This study was also undertaken to test the in vitro antimicrobial activity with the extracts of Dansam-samultang. In general, the DSE showed the significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermiders and Escherichia coli. In single oral dose toxicity study, in vivo, there were no differences between control and treated groups in clinical signs, body weight gains, and gross finding. The results indicated that DSE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of DSE was found to be higher than 10 mL/kg in this experiment. In conclusion, the extracts from Dansam-samultang may act as a natural subsistence for functional cosmetics.