Human umbilical cord mesenchymal stem cells co-culture ameliorates the innate immunity of podocytes induced by high glucose / 中华肾脏病杂志

ABSTRACT

Objective To explore the effects of human umbilical cord mesenchymal stem cells (HUC-MSCs) on the innate immunity of podocytes mediated by Toll-like receptor (TLR)signaling pathway under high glucose (HG) condition.Methods Podocytes were divided into four groups according to the treatmentnormal glucose group (NG),mannitol control group (NG+MA),high glucose group (HG) and HUC-MSCs co-culture group (HG+MSC).After 72 hours treatment,the protein levels of interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α),heat shock protein 70 (HSP70),high-mobility group box-1 (HMGB1)in culture medium were measured by ELISA.Real-time PCR was used to detect the mRNA expressions of TLR2 and TLR4.Western blotting was used to detect the protein expressions of TLR2,TLR4,myeloid differentiation factor 88 (MyD-88) and phospho-P65 (p-P65).Immunofluorescence staining was used to study the localization of p-P65 in podocytes.Results High glucose induced the inflammation of podocytes by activating the TLR signaling,which increased the secretion of IL-6,TNF-α,HSP70,HMGB1,the mRNA level of TLR2,TLR4 and the protein level of TLR2,TLR4,MyD-88 and p-P65 (all P ＜ 0.05).High glucose also activated NF-κB and induced its nuclear translocation.HUC-MSCs co-culture decreased the inflammation and restrained the TLR signaling.Conclusions HUC-MSCs co-culture decreases the inflammation and innate immunity of podocytes induced by HG.