Establishment and Evaluation of Fingerprint Identification Pattern for Epimedium Pieces / 中国药师

ABSTRACT

Objective:To establish a comprehensive quality evaluation pattern for epimedium pieces to provide reference for the quality control. Methods:The overall quality of Epimedium brevicornu Maxim was evaluated through the establishment of a fingerprint identification mode to perform the bifunctional feedback to the fingerprint peaks. The chromatographic separation was carried out on an Agilent ZORBAX Eclipse XDB-C18 (150 mm × 4. 6 mm,5 μm) column with the mobile phase of acetonitrile and water with gradient e-lution. The flow rate was 1. 0 ml·min1 , and the column temperature was maintained at 30℃. Results:The HPLC fingerprint of Epi-medium brevicornu Maxim decoction pieces containing 16 common peaks was obtained. The marketed Epimedium brevicornu Maxim de-coction pieces were divided into two categories and the quality between them showed significant differences. Conclusion:The similarity of 11 batches of epimedium herbs used to establish the fingerprints shows notable different, and the different habitats and sampling time exhibit significant influence on the overall quality. The fingerprint pattern can effectively reflect the overall trend of quality of Chinese herbal pieces, which is suitable for comprehensive quality evaluation of traditional Chinese medicines with multiple components and their relative preparations.