Determination and Analysis of Kaempferol in Sedum aizoon L.by HPLC-MS/MS / 中华中医药杂志
Article
en Zh
| WPRIM
| ID: wpr-679843
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To discover and determine the content of kaempferol in Sedum aizoon L.for the first time.Methods:
Waters HPLC-MS/MS,XTerra-MS C18 (5?m,2.1?150mm) and the mobile phase consisted of acetonitrile-water-formic(40∶60∶1) were applied to find kaempferol in Sedum aizoon L.;Daojin HPLC and the SHIM-PACK VP C18(250nm?4.6nm,10?m) column were used.The mobile phase consisted of methanol-0.4% phosphoric acid solutions(5941) with the flow rate at 1.0ml/min and the UV detector wave-length were set at 370nm.Results:
Compared with standard sample,the thing that kaempferol exists in Sedum aizoon L.was confirmed.The calibration curve was in good linearity over the range of 2.0-8.0?g,and regression equation was Y=40343X-11107(r=0.9998).The average recovery rate was 102.53%,with RSD =0.92%(n=6).Conclusion:
The method is simple,accurate and reproducible so it can be used to determine and analyze the content of kaempferol in Sedum aizoon L.
Texto completo:
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
China Journal of Traditional Chinese Medicine and Pharmacy
Año:
2005
Tipo del documento:
Article