Establishment of cloned homogeneous immunoassay for detection of P53 protein in serum and stool samples from patients with gastric carcinoma / 中华检验医学杂志

ABSTRACT

Objective To evaluate reliability of the cloned homogeneous immunoassay technique for detection of P53 protein content in serum and stool from patients with gastric carcinoma. Methods To prepare EA, ED and ED P53 fusion protein of beta galactosidase by PGEX 4T 2 expression vector, a novel cloned homegeneous immunoassay was establishment for detection of P53 protein in serum and stool samples from patients with gastric carcinoma and non gastric carcinoma groups as well as healthy control. Results The concentration of P53 protein in 31 gastric carcinoma subjects were remarkably increased as compared with 56 non gastric carcinoma ( P