Cloning and Alignment of the Partial Mannanase Gene of Bacillus spp / 微生物学通报
Microbiology
; (12)1992.
Article
en Zh
| WPRIM
| ID: wpr-685307
Biblioteca responsable:
WPRO
ABSTRACT
By functional plates,16 strains which can produce?-mannana-se were isolated frnm 28 Bacillus spp.Using a pair of degenerated primers,the conserved fragments of?-mannanase gene from the selected strains were amplified by PCR.The obtained nucleotide fragments were sequenced and compared with the homologous?-mannanase genes in GenBank and a phylogenetic tree was generated.Comparing to the genes coding?-mannanase published,the cloned nucleotide fragments show the highest sequence identity between 62% and 98%.The genes coding fnr?-mannanase of Bacillus circulus have low identity while the?-mannanase genes of Bacillus subtilis and other Bacillus spp. have high identity.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Microbiology
Año:
1992
Tipo del documento:
Article