Hepatocytes apoptosis induced by β1-adrenoceptor autoantibody is involved in hepatic dysfunction / 基础医学与临床

ABSTRACT

Objective To investigate the effect of β1-Adrenoceptor autoantibody on liver function.Methods The biologically active of β1-AA was prepared and passive immunization model was established with β1-AA.The bio-chemical parameters of the liver were measured by the automatic serum biochemical analyzer.The liver size, hepatic vein,portal vein velocity were detected by liver ultrasound;hepatocytes apoptosis were tested by tunel stai-ning,annexin V/PI staining and caspase 3 activity detection.Results The biologically active of β1-AA and passive immunization model were established successfully.The ALT and AST of the liver significantly increased and the ALB decreased in the passive immunization process.The apoptosis of the hepatocytes increased,and meto-prolol partially reversed this effect.Conclusions β1-AA may induce hepatocytes apoptosis by β1-adrenergic receptor and participate in the development of liver injury.