A method of establishing type 2 diabetic cardiomyopathy model in mice / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 764-768, 2018.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-701193
Biblioteca responsable:
WPRO
ABSTRACT
AIM:
To establish a mouse model of type 2 diabetic cardiomyopathy induced by sustained high-fat diet(HFD)feeding and single intraperitoneal injection of streptozotocin(STZ).METHODS:
The 5 ~6-week-old C57BL/6J mice were randomly divided into 2 groups(n=20 per group) the mice in control group received a sustained regular diet;the mice in HFD+STZ group received a sustained HFD and were intraperitoneally injected with STZ(100 mg/kg)at the 5th week.The body weight and blood glucose were measured at 0,5,6,11 and 16 weeks.The mice in control group and HFD +STZ group were analyzed with echocardiography,HE staining,ELISA and immunohistochemistry at the 11th and 16th weeks.RESULTS:
The body weight of the mice in HFD +STZ group was higher than that in control group(P<0.05)during each modeling period,and was slightly decreased 1 week after STZ injection.The blood glucose of the mice in HFD+STZ group was higher than 13.89 mmol/L 1 week after STZ injection.The serum insulin of the mice in HFD+STZ group was lower than that in control group(P<0.05)at the 11th week and the 16th week.Echocardio-graphy, HE staining and immunohistochemistry analysis showed that the mice in HFD +STZ group had mild ventricular dysfunction and cardiomyocyte hypertrophy, and cardiomyocyte area and apoptosis rate were obvious higher than those in control group(P<0.05)at the 16th week,while these indicators were not obviously changed in HFD +STZ group com-pared with control group at the 11th week.CONCLUSION:
The sustained HFD feeding and single intraperitoneal injec-tion of STZ successfully establish a mouse model of type 2 diabetic cardiomyopathy.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
2018
Tipo del documento:
Artículo