Regulation Effect of Berberine on the Inhibition of Hexokinase Ⅱ in Breast Cancer Cells Glycolysis / 中国药师

ABSTRACT

Objective:To investigate the effects of berberine on glycolysis in breast cancer cells and its effect on hexokinase Ⅱ. Methods:The inhibitory effects of berberine on the proliferation of breast cancer cells were studied by MTT with human breast cancer cell line MDA-MB-231 and MCF-7. The glucose consumption and lactic acid in breast cancer cells were detected to evaluate the effect of berberine on glycolysis in breast cancer cells. The energy supply situation of breast cancer cells was evaluated after the detection of ATP and NAD+/NADH. Finally,the hexokinase Ⅱ activity and protein content were also detected in breast cancer cells.Results:The experimental results showed that berberine had an obvious inhibitory effect on the proliferation of human breast cancer cell line MDA-MB-231 and MCF-7 cell in a concentration-dependent manner. While reducing the cell ATP content and increasing NAD+/NADH con-tent(P<0.05),berberine could clearly reduce the consumption of glucose and lactic acid content in the different breast cancer cell lines. In addition,berberine could inhibit the activity and protein content of hexokinase Ⅱ in breast cancer cells.Conclusion:Berber-ine can significantly inhibit the proliferation and the glycolysis of breast cancer cells,reduce the energy supply and obviously inhibit the expression of hexokinase Ⅱ in breast cancer cells as well.