Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice / 대한배뇨장애요실금학회지
International Neurourology Journal
; : S139-S146, 2018.
Article
en En
| WPRIM
| ID: wpr-717677
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice.METHODS:
For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed.RESULTS:
Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment.CONCLUSIONS:
In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Privación de Sueño
/
Triptófano Hidroxilasa
/
Tirosina 3-Monooxigenasa
/
Inmunohistoquímica
/
Agua
/
Dopamina
/
Serotonina
/
Regulación hacia Arriba
/
Western Blotting
/
Receptores Dopaminérgicos
Límite:
Animals
Idioma:
En
Revista:
International Neurourology Journal
Año:
2018
Tipo del documento:
Article