Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
The Korean Journal of Physiology and Pharmacology
; : 555-566, 2018.
Article
en En
| WPRIM
| ID: wpr-727868
Biblioteca responsable:
WPRO
ABSTRACT
Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and β-catenin; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Regeneración
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Células Madre
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Cordón Umbilical
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Técnicas In Vitro
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Factor I del Crecimiento Similar a la Insulina
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Supervivencia Celular
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Proteína 1 de Unión a Factor de Crecimiento Similar a la Insulina
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Folículo Piloso
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Técnicas de Cocultivo
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Péptidos y Proteínas de Señalización Intercelular
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2018
Tipo del documento:
Article