High glucose and palmitate increases bone morphogenic protein 4 expression in human endothelial cells
The Korean Journal of Physiology and Pharmacology
; : 169-175, 2016.
Article
en En
| WPRIM
| ID: wpr-728538
Biblioteca responsable:
WPRO
ABSTRACT
Here, we investigated whether hyperglycemia and/or free fatty acids (palmitate, PAL) aff ect the expression level of bone morphogenic protein 4 (BMP4), a proatherogenic marker, in endothelial cells and the potential role of BMP4 in diabetic vascular complications. To measure BMP4 expression, human umbilical vein endothelial cells (HUVECs) were exposed to high glucose concentrations and/or PAL for 24 or 72 h, and the effects of these treatments on the expression levels of adhesion molecules and reactive oxygen species (ROS) were examined. BMP4 loss-of-function status was achieved via transfection of a BMP4-specific siRNA. High glucose levels increased BMP4 expression in HUVECs in a dose-dependent manner. PAL potentiated such expression. The levels of adhesion molecules and ROS production increased upon treatment with high glucose and/or PAL, but this eff ect was negated when BMP4 was knocked down via siRNA. Signaling of BMP4, a proinflammatory and pro-atherogenic cytokine marker, was increased by hyperglycemia and PAL. BMP4 induced the expression of infl ammatory adhesion molecules and ROS production. Our work suggests that BMP4 plays a role in atherogenesis induced by high glucose levels and/or PAL.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Transfección
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Especies Reactivas de Oxígeno
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ARN Interferente Pequeño
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Células Endoteliales
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Diabetes Mellitus
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Angiopatías Diabéticas
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Aterosclerosis
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Ácidos Grasos no Esterificados
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Células Endoteliales de la Vena Umbilical Humana
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Glucosa
Límite:
Humans
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2016
Tipo del documento:
Article