Identification of Epithelial-Mesenchymal Transition-related Target Genes Induced by the Mutation of Smad3 Linker Phosphorylation
Journal of Cancer Prevention
; : 1-9, 2018.
Article
en En
| WPRIM
| ID: wpr-740095
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Smad3 linker phosphorylation plays essential roles in tumor progression and metastasis. We have previously reported that the mutation of Smad3 linker phosphorylation sites (Smad3-Erk/Pro-directed kinase site mutant constructs [EPSM]) markedly reduced the tumor progression while increasing the lung metastasis in breast cancer. METHODS: We performed high-throughput RNA-Sequencing of the human prostate cancer cell lines infected with adenoviral Smad3-EPSM to identify the genes regulated by Smad3-EPSM. RESULTS: In this study, we identified genes which are differentially regulated in the presence of Smad3-EPSM. We first confirmed that Smad3-EPSM strongly enhanced a capability of cell motility and invasiveness as well as the expression of epithelial-mesenchymal transition marker genes, CDH2, SNAI1, and ZEB1 in response to TGF-β1 in human pancreatic and prostate cancer cell lines. We identified GADD45B, CTGF, and JUNB genes in the expression profiles associated with cell motility and invasiveness induced by the Smad3-EPSM. CONCLUSIONS: These results suggested that inhibition of Smad3 linker phosphorylation may enhance cell motility and invasiveness by inducing expression of GADD45B, CTGF, and JUNB genes in various cancers.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Neoplasias Pancreáticas
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Fosforilación
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Fosfotransferasas
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Neoplasias de la Próstata
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Neoplasias de la Mama
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Línea Celular
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Movimiento Celular
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Análisis de Secuencia de ARN
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Transición Epitelial-Mesenquimal
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Pulmón
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Journal of Cancer Prevention
Año:
2018
Tipo del documento:
Article