Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis
The Korean Journal of Parasitology
; : 113-119, 2018.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-742242
Biblioteca responsable:
WPRO
ABSTRACT
Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20–40 year-old group, followed by 40–60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.
Texto completo:
Disponible
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Enfermedades Desatendidas
/
Zoonosis
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Ensayo de Inmunoadsorción Enzimática
/
Agua
/
Factores Epidemiológicos
/
Reacción en Cadena de la Polimerasa
/
Epidemiología
/
Criptosporidiosis
/
Cryptosporidium
/
Oocistos
/
Diagnóstico
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
/
Humanos
País/Región como asunto:
Africa
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2018
Tipo del documento:
Artículo