Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes
Journal of Cancer Prevention
; : 123-128, 2019.
Article
en En
| WPRIM
| ID: wpr-764303
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes. METHODS: Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H₂O₂. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay. RESULTS: Esculetin prevented H₂O₂-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity. CONCLUSIONS: Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Piel
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Envejecimiento
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ARN Mensajero
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Queratinocitos
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Western Blotting
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Calcio
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Especies Reactivas de Oxígeno
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Factor de Transcripción AP-1
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Estrés Oxidativo
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Metaloproteinasa 1 de la Matriz
Límite:
Humans
Idioma:
En
Revista:
Journal of Cancer Prevention
Año:
2019
Tipo del documento:
Article