Inducing Effect of Decitabine on Apoptosis of KMS-18 Myeloma Cells and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1149-1153, 2019.
Article
en Zh
| WPRIM
| ID: wpr-775750
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of decitabine on proliferation and apoptosis of multiple myeloma KMS-18 cells and its possible mechanism.@*METHODS@#CCK-8 was used to detect cell proliferation, flow cytometry was used to detect the changes of apoptosis, real-time quantitative PCR was used to detect the expression of P53 gene mRNA in myeloma KMS-18 cells, and MSP assay was used to detect the methylation status of P53 gene promoter.@*RESULTS@#The proliferation inhibition and apoptosis of KMS-18 cells significantly increased after treatment by decitabine (P<0.05). The expression of P53 mRNA increased in KMS-18 cells after treatment of decitabine (P<0.05). The methylation status of the P53 gene promoter in KMS-18 cells could be partially reversed by decitabine.@*CONCLUSION@#Decitabine can inhibit the proliferation of KMS-18 cells and induce their apoptosis, its mechanism ralates with partially reversing the methylation of P53 gene promoter in KMS-18 cells.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Apoptosis
/
Metilación de ADN
/
Línea Celular Tumoral
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Proliferación Celular
/
Decitabina
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2019
Tipo del documento:
Article