Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent / 대한소아치과학회지
Journal of Korean Academy of Pediatric Dentistry
; (4): 32-40, 2018.
Article
en Ko
| WPRIM
| ID: wpr-787299
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus spp. quantified using a quantitative real-time polymerase chain reaction (qRT-PCR).15 healthy children of ages 9 – 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species.The levels of S. mutans, S. sobrinus, and Lactobacillus spp. were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque.The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans, S. sobrinus, and Lactobacillus spp.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Streptococcus mutans
/
Bacterias
/
Diente
/
ADN
/
Streptococcus sobrinus
/
Caries Dental
/
Placa Dental
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Lactobacillus
Límite:
Child
/
Humans
Idioma:
Ko
Revista:
Journal of Korean Academy of Pediatric Dentistry
Año:
2018
Tipo del documento:
Article