The chemical constituents and antioxidant activity of Ixeris sonchi f olia / 药学实践杂志

ABSTRACT

Objective To study thechemicalconstituentsof Ixerissonchifolia.Methods Monomericcompoundswerei‐solated by chromatography on silica gel column ,Sephadex LH‐20 ,ODS chromatography column and HPLC .Thestructure of the compound wasconfirmed on the basis of physio‐chemical constants and spectroscopic analysis .2 ,2‐diphenyl‐1‐pikrylhydra‐zyl (DPPH) and 3‐ethylbenzthiazoline‐6‐sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds .Results Five compounds were isolated from 70% ethanol‐water extraction and their structures were identified as (+ )‐pinoresinol‐4‐O‐β‐D‐glucopyranoside(1) ,glochidioboside(2) ,3 ,4 ,5‐trimethoxyphenyl‐1‐O‐β‐D‐glucopy‐ranoside(3) ,on‐onin(4) ,daidzein(5) ,luteolin (6) ,and apigenin (7) .Meanwhile ,antioxidant activities of all the isolates were evaluated by DPPHand ABTS .Conclusion Compounds 1‐5 were isolated from this genus for the first time .The active results showed that 1 ,2 ,4‐7 exhibited potent antioxidant activity .