Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 353-354,378, 2017.
Article
en Zh
| WPRIM
| ID: wpr-790769
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji.Methods The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile and 0.05 mol/L NaH2PO4 solution(pH was adjusted to about 3 with phosphoric acid).The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride.Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6).The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5).Conclusion This method is accurate, sensitive, selective and reproducible.It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice
Año:
2017
Tipo del documento:
Article