Evaluation of HPLC Fingerprint of Lonicerae Japonicae Flos and Lonicerae Flos Based on Total Statistical Moment Analysis Method / 中国实验方剂学杂志

ABSTRACT

Objective: To evaluate the HPLC fingerprints of Lonicerae Japonicae Flos and Lonicerae Flos by total statistical moment analysis,in order to provide a basis for studying the nature of the "heterologous effect" phenomenon. Method: HPLC fingerprints of Lonicerae Japonicae Flos and Lonicerae Flos were established,and the statistical moment parameters and similarity were evaluated by total statistical moment method. Result: According to the total statistical moment parameters of the 10 batches of Lonicerae Japonicae Flos samples, RSDs of AUCT,λT, σT2 were 27.537%,1.685%,and 8.346%. According to the total statistical moment parameters of the 10 batches of Lonicerae Flos samples, RSDs of AUCT, λT, σT2 were 14.752%,2.155% and 2.882%. The similarity of 10 batches of Lonicerae Japonicae Flos fingerprints was above 0.92,and the similarity of 10 batches of Lonicerae Flos were above 0.93. The 10 batches of Lonicerae Japonicae Flos and the 10 batches of Lonicerae Flos fingerprints were compared,and the similarity was above 0.84. Conclusion: According to the results,the similarity of the Lonicerae Japonicae Flos and Lonicerae Flos fingerprints was very high,which indicated a similarity in chemical composition and composition ratio between Lonicerae Japonicae Flos and Lonicerae Flos. This may be a prerequisite for the phenomenon of "heterologous effects".