Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters / 中华男科学杂志
National Journal of Andrology
; (12): 329-336, 2017.
Article
en Zh
| WPRIM
| ID: wpr-812764
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#Sperm DNA fragmentation (SDF) is widely used to predict male infertility and the methods of detecting SDF are varied. This study aimed to compare two methods of SDF detection and investigate the correlation between SDF and sperm quality.@*METHODS@#Using sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD), we detected SDF in 108 semen samples collected in the Center of Reproduction and Genetics of Suzhou Municipal Hospital. We compared the results of the two methods and analyzed the correlations of SDF routine semen parameters, sperm morphology and the age of the patients.@*RESULTS@#A significant consistency was found in the SDF index (DFI) between the two methods (P<0.01). The DFI was correlated negatively with sperm motility, the percentage of progressively motile sperm, and that of morphologically normal sperm (P <0.01), but positively with the teratozoospermia index (P <0.01 in SCSA and P <0.05 in SCD). The DFI measured by SCSA showed a significantly positive correlation with the patients' age (P <0.01), but not that obtained by SCD.@*CONCLUSIONS@#The results of both SCSA and SCD play an important role in predicting sperm quality. As a clinical index, the DFI has a predictive value for male infertility. However, the results of different detecting methods vary widely, which calls for further studies on their standardization.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Semen
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Motilidad Espermática
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Espermatozoides
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Cromatina
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Diagnóstico
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Fragmentación del ADN
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Análisis de Semen
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Genética
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Infertilidad Masculina
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2017
Tipo del documento:
Article