Appropriate In Vitro Methods for Genotoxicity Testing of Silver Nanoparticles
Environmental Health and Toxicology
; : e2013003-2013.
Article
en En
| WPRIM
| ID: wpr-81327
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVES:
We investigated the genotoxic effects of 40-59 nm silver nanoparticles (Ag-NPs) by bacterial reverse mutation assay (Ames test), in vitro comet assay and micronucleus (MN) assay. In particular, we directly compared the effect of cytochalasin B (cytoB) and rat liver homogenate (S9 mix) in the formation of MN by Ag-NPs.METHODS:
Before testing, we confirmed that Ag-NPs were completely dispersed in the experimental medium by sonication (three times in 1 minute) and filtration (0.2 microm pore size filter), and then we measured their size in a zeta potential analyzer. After that the genotoxicity were measured and especially, S9 mix and with and without cytoB were compared one another in MN assay.RESULTS:
Ames test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains revealed that Ag-NPs with or without S9 mix did not display a mutagenic effect. The genotoxicity of Ag-NPs was also evaluated in a mammalian cell system using Chinese hamster ovary cells. The results revealed that Ag-NPs stimulated DNA breakage and MN formation with or without S9 mix in a dose-dependent manner (from 0.01 microg/mL to 10 microg/mL). In particular, MN induction was affected by cytoB.CONCLUSIONS:
All of our findings, with the exception of the Ames test results, indicate that Ag-NPs show genotoxic effects in mammalian cell system. In addition, present study suggests the potential error due to use of cytoB in genotoxic test of nanoparticles.Palabras clave
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Base de datos:
WPRIM
Asunto principal:
Ovario
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Salmonella typhimurium
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Plata
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Sonicación
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ADN
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Pruebas de Micronúcleos
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Cricetulus
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Ensayo Cometa
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Citocalasina B
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Nanopartículas
Límite:
Animals
Idioma:
En
Revista:
Environmental Health and Toxicology
Año:
2013
Tipo del documento:
Article